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accessory protein expression vectors  (Addgene inc)


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    Addgene inc accessory protein expression vectors
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Protein Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accessory protein expression vectors/product/Addgene inc
    Average 93 stars, based on 3 article reviews
    accessory protein expression vectors - by Bioz Stars, 2026-05
    93/100 stars

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    1) Product Images from "SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways"

    Article Title: SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways

    Journal: ACS Omega

    doi: 10.1021/acsomega.4c02631

    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Figure Legend Snippet: SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Techniques Used: Western Blot, Expressing, Transfection, Plasmid Preparation, Control



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    accessory protein expression vectors  (Addgene inc)
    93
    Addgene inc accessory protein expression vectors
    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 <t>expression.</t> A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression <t>vectors</t> and then lysed for <t>protein</t> detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.
    Accessory Protein Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/accessory protein expression vectors/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    accessory protein expression vectors - by Bioz Stars, 2026-05
    93/100 stars
    		  Buy from Supplier

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    SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Journal: ACS Omega

    Article Title: SARS-CoV-2 Protein Nsp9 Is Involved in Viral Evasion through Interactions with Innate Immune Pathways

    doi: 10.1021/acsomega.4c02631

    Figure Lengend Snippet: SARS-CoV-2 Nsp9 Western blot analysis. A) SARS-CoV-2 Orf6, Orf3a, and Nsp9 expression. A549 cells were reverse transfected with 2 μg Orf6, Orf3a, or Nsp9 expression vectors and then lysed for protein detection by Western blotting. B) Western blotting of p-NF-kB, NF-kB, and TBK1 protein expression in 24 h poly I:C-treated A549 cells transfected with Nsp9 or empty vector control plasmids. B-tubulin is used as a control for protein expression. C) Densitometry analysis showing relative TBK1 abundance in 24 h poly I:C (PIC)-treated A549 cells transfected with Nsp9 or empty vector control plasmids compared to nontransfected and nontreated A549 cells ( n = 3). Error bars represent standard error of the mean, * = p < 0.05.

    Article Snippet: SARS-CoV-2 nonstructural and accessory protein expression vectors were purchased from Addgene (MA, USA): pTwist-CMV-Nsp9–3xFlag (pGBW- m4133209), pTwist-CMV-ORF6–3xFlag (pGBW-m4252831), and pTwist-CMV-ORF3a- 3xFlag (pGBW-m4133902).

    Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Control